Callose deposition in infected sorghum and maize. Sorghum (A, B, E) and maize (C, D, F) leaves were collected at 2 days after inoculation (dai). Sorghum infected with S
porisorium reilianum f. sp. reilianum (SRS) showed weak and localized callose deposition (A, E), whereas stronger and more frequent levels of callose were observed for S
. reilianum f. sp. zeae (SRZ) (B, E). In maize, callose was observed in small and localized amounts for SRS (C) and SRZ (D), and was slightly more frequent for SRZ (F). (E) and (F) show the percentage of occurrence of callose at hyphal cell‐to‐cell crossings in sorghum and maize, respectively. Hyphal cell‐to‐cell crossings were counted for three biological replicates consisting of two leaves of two plants each. Data given are the mean values and standard deviations. The total number of counted cell‐to‐cell crossings is given above the bars. Different letters above the columns indicate significant differences (t‐test with P ≤ 0.05).