Table 4.
P ratylenchus zeae transcripts with putative homology to those of proteins/peptides of the secretomes of M eloidogyne incognita and H eterodera glycines and secretory–excretory products of B rugia malayi
| Secretome of nematode | ||||||
|---|---|---|---|---|---|---|
| Secretome of sedentary endoparasites | Excretory–secretory products of B. malayi life stages | |||||
| Proteins | M. incognita * | H. glycines † | Host–parasite interface‡ | Microfilariae, female and males§ | Adult life stage¶ | Number of matching contigs (singletons) |
| Structural/cytoskeleton/nuclear | ||||||
| Actin | YES | — | — | — | YES | 13 (186) |
| Calmodulin | YES | — | — | — | YES | 9 (26) |
| Tropomyosin family protein | YES | — | — | — | YES | 6 (120) |
| High‐mobility group (HMG) protein | YES | — | — | — | YES | 10 (37) |
| Intermediate filament protein | — | — | YES | — | — | 3 (12) |
| Cuticle collagen | — | — | YES | — | — | 8 (46) |
| N‐Heparan sulfate | — | — | YES | — | — | 0 (0) |
| Troponin C‐like protein | YES | — | — | — | — | 2 (0) |
| Myosin regulatory light chain | YES | — | — | — | — | 7 (72) |
| Polygalacturonase | YES | — | — | — | — | 0 (1) |
| Cellulose‐binding protein | YES | YES | — | — | — | 1 (1) |
| Twitchin (unc‐22) | YES | — | YES | — | — | 2 (42) |
| Guanylyl cyclase | — | YES | — | — | — | 2 (47) |
| Chitinase | — | YES | — | — | — | 0 (8) |
| Histone H4 | — | ‐ | YES | — | — | 1 (14) |
| Msp‐1 | — | YES | — | YES | YES | 2 (13) |
| Energy metabolism | ||||||
| 6‐Phosphofructokinase | — | — | — | — | YES | 0 (2) |
| Enolase | YES | — | — | YES | YES | 1 (11) |
| Fructose‐bisphosphate aldolase 1 | — | — | — | YES | — | 0 (0) |
| Transaldolase | — | — | YES | — | — | 1 (5) |
| ATP synthase (atp‐2) | YES | — | ‐ | — | — | 17 (72) |
| Adenylate cyclase | — | — | YES | — | — | 2 (9) |
| Nucleoside diphosphate kinase | — | — | YES | — | — | 3 (6) |
| Triosephosphate isomerase | YES | — | YES | YES | YES | 1 (1) |
| Guanylate kinase | — | — | YES | — | — | 8 (11) |
| Phosphoglycerate mutase | — | — | — | YES | — | 1 (4) |
| Protein digestion, folding and fate/calcium binding | ||||||
| Aminopeptidase | — | — | YES | YES | — | 3 (20) |
| Calreticulin | YES | — | — | — | YES | 0 (6) |
| Serine carboxypeptidase | — | — | — | — | YES | 2 (10) |
| Ubiquitin (extension protein) | YES | YES | — | — | YES | 0 (0) |
| Ubiquitin‐like protein SMT3 | YES | — | — | — | YES | 2 (8) |
| Peptidyl‐prolyl cis–trans isomerase | YES | — | YES | — | YES | 3 (23) |
| Serine/threonine protein phosphatase | — | — | YES | — | YES | 9 (72) |
| Cytochrome c type 1 protein | — | — | YES | — | — | 2 (0) |
| SKP‐1 | — | YES | — | — | — | 3 (22) |
| Putative trypsin inhibitor | — | — | — | YES | — | 0 (13) |
| Calsequestrin family protein | — | — | — | YES | YES | 1 (5) |
| Cyclophilin | YES | — | — | — | YES | 3 (28) |
| Protein disulfide isomerase | — | — | — | — | YES | 4 (10) |
| Cytosol stress response and antioxidants | ||||||
| 14‐3‐3b | YES | — | — | — | YES | 5 (24) |
| Hsp 70 | YES | — | — | — | YES | 3 (9) |
| Heat shock 90 | — | — | — | YES | ‐ | 5 (26) |
| Glutathione peroxidase | YES | — | YES | YES | YES | 4 (16) |
| Thioredoxin | — | — | YES | — | YES | 3 (14) |
| Superoxide dismutase | YES | — | YES | YES | YES | 2 (16) |
| Translationally controlled tumour protein | YES | — | — | — | YES | 2 (1) |
| Annexin | YES | YES | — | — | — | 1 (17) |
| Glutathione‐S‐transferase | YES | — | — | — | — | 3 (12) |
| Glutathione reductase | — | — | YES | — | — | 3 (0) |
| Glutathione synthetase | YES | — | — | — | — | 3 (6) |
| — | — | — | — | — | ||
| Lectins and glycosyltransferases | ||||||
| Galectin | YES | — | YES | YES | YES | 7 (33) |
| N‐Acetylglucosaminyltransferase | — | — | — | — | YES | 2 (9) |
| C‐type lectin | — | — | — | — | YES | 1 (0) |
| Protease inhibitors | ||||||
| Cystatin‐type cysteine proteinase inhibitor | YES | — | YES | YES | YES | 3 (1) |
| Lipid binding | ||||||
| Phosphatidylethanolamine‐binding protein | — | — | YES | — | YES | 1 (10) |
| Fatty acid retinol‐binding protein | YES | — | — | — | — | 2 (8) |
| Other proteins | ||||||
| TTLs | YES | — | YES | — | YES | 9 (36) |
| Venom allergen‐like proteins | YES | — | YES | — | ‐ | 0 (1) |
| Mucin‐like protein | — | — | — | — | YES | 1 (3) |
| Host cytokine homologues | ||||||
| Macrophage migration inhibitory factor 1 (MIF‐1) | — | — | YES | YES | YES | 0(0) |
| Plant cell wall degradation enzymes | ||||||
| Pectate lyase | YES | YES | — | — | — | 0 (1) |
| Endoglucanase (and precursors) | YES | YES | — | — | — | 1 (27) |