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. 2016 Apr 13;17(9):1364–1381. doi: 10.1111/mpp.12367

Figure 7.

Figure 7

Real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) and electrophoretic mobility shift assay (EMSA) of fungal DHN‐melanin biosynthesis‐related genes. (A) RT‐PCR analysed the expression of Pks1, Brn1, Brn2 and Scd1 at 0, 18, 48 and 120 h. WT, wild‐type; KO, knockout mutants; IM, insertion mutants; Com, complemented strains. (B) EMSA for Vdpf (EGY18187.1) and the promoters of melanin synthesis‐related genes. The promoters of Brn1, Brn2, Pks1 and Scd1 were used for the binding assays with 0, 3, 4 or 5 μL of purified Vdpf. The concentration of purified Vdpf was 513.636 μg/mL. The red arrows point to the shift bands. Four biological replicates (n = 4) were used for this study.