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. 2016 Mar 6;17(6):973–984. doi: 10.1111/mpp.12351

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© 2015 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Graft inoculation of SC12‐1‐99 (scion) on infected CMV‐LS Piel de Sapo (PS) (rootstock). Grafts are represented as the rootstock under the grafted junction (white arrow) and the scion above the junction. (a) Representative healthy, asymptomatic graft. Detection of virus was carried out by tissue printing on distal to proximal leaves from the graft (numbers 1–5), showing that no virus was present in the scion. C+, leaf from CMV‐LS‐infected PS plant. (b) Graft showing very mild symptoms in proximal leaves. Virus was detected by tissue printing on distal to proximal leaves from the graft (numbers 1–5), showing virus in proximal leaves only (leaf 5 and less virus in leaf 4). CMV, Cucumber mosaic virus.