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. 2014 Jun 30;16(1):61–70. doi: 10.1111/mpp.12163

Figure 3.

figure

PsMPK7‐silenced transformants did not suppress host callose deposition or the reactive oxygen species (ROS) burst. (A) Etiolated soybean seedlings were infected with zoospores and stained with aniline blue after 16 h. The epidermal cells were peeled for microscopic observation. Callose in the host tissues beneath the zoospore penetration sites was seen as fluorescent solid circular shapes (see the corresponding light microscopic fields). Bar represents 50 μm. (B) 3,3′‐Diaminobenzidine staining of soybean seedlings infected by PsMPK7‐silenced transformants and the wild‐type (WT) after 10 h. Epidermal cells were peeled for microscopic observation. The penetration sites are indicated by red arrows. Bar represents 50 μm.