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. 2015 Mar 16;16(9):921–930. doi: 10.1111/mpp.12220

Figure 5.

figure

Effects of NbFib2 depletion on Rice stripe virus (RSV) infection. NbFib2‐silenced Nicotiana benthamiana plants belonging to Group II were inoculated with RSV (A, left), Potato virus X‐green fluorescent protein (PVX‐GFP) (B, right) or Tobacco mosaic virus‐GFP (TMV‐GFP) (B, left), as described in the text. Nicotiana benthamiana previously inoculated with empty Tobacco rattle virus (TRV) was used as a control (A, right). Nicotiana benthamiana plants are photographed at 22 days after virus inoculation. The accumulation of RSV 22 days after virus inoculation in inoculated (In) or upper (Sys) leaves was detected by reverse transcription‐polymerase chain reaction (RT‐PCR, C, left) and Western blotting (C, right). The presence of PVX‐GFP and TMV‐GFP was visualized under UV light (B), and the presence of intact PVX and TMV virions in systemically infected leaves was demonstrated by electron microscopy (D; scale bars, 100 nm).