Figure 1.
Toxoflavin production by Burkholderia. Five wild‐type strains of Burkholderia were tested: B. glumae BGR1, B. gladioli BSR3, B. gladioli KACC11889, B. gladioli KCTC12347 and B. gladioli KACC13944. The supernatant of the bacterial culture was extracted with chloroform and the mixture was centrifuged; the resulting infranatant was dried in a centrifugal vaporizer. The solutes were separated in 80% methanol on a thin‐layer chromatography (TLC) silica gel plate. The toxoflavin produced was examined visually in daylight and under UV light at 365 nm (T, toxoflavin). The origin (o) and solvent front (f) on the TLC plate are shown.