Table 2.
Bacterial strains and plasmids used in this study
| Name | Description | Source |
|---|---|---|
| Bacterial strains | ||
| BGR1 | Burkholderia glumae isolate from rice, wild‐type | Jeong et al. (2003) |
| BGS2 | BGR1 tofI::Ω | Kim et al. (2004) |
| BSR3 | B. gladioli isolate from rice, wild‐type | Seo et al. (2011) |
| COK94 | BSR3 tofI::lacZ | Laboratory collection |
| KACC11889 | B. gladioli isolate from gladiolus, wild‐type | KACC |
| KCTC12374 | B. gladioli isolate from onion, wild‐type | KCTC |
| KACC13944 | B. gladioli isolate from mushroom, wild‐type | KACC |
| 11889EMPTY | KACC11889 transformed with empty vector pBBR1MCS‐2 | This study |
| 11889ATOFI | KACC11889 transformed with pATOFI | This study |
| 11889ATOFM | KACC11889 transformed with pATOFM | This study |
| 11889ATOFR | KACC11889 transformed with pATOFR | This study |
| 11889ATOFIMR | KACC11889 transformed with pATOFIMR | This study |
| 11889UTOFI | KACC11889 transformed with pUTOFI | This study |
| 11889UTOFM | KACC11889 transformed with pUTOFM | This study |
| 11889UTOFR | KACC11889 transformed with pUTOFR | This study |
| 11889UTOFIMR | KACC 11889 transformed with pUTOFIMR | This study |
| DH5α λpir | Escherichia coli used for cloning and propagation of plasmids | Laboratory collection |
| S17‐1 λpir | E. coli used for analysis of conjugal transfer functions | Kalogeraki and Winans (1997) |
| Plasmids | ||
| pBBR1MCS‐2 | Broad‐host‐range vector containing mobilization gene and kanamycin resistance gene | Kovach et al. (1995) |
| pATOFI | pBBR1MCS‐2 containing bgla_2g11050 (tofI) gene of BSR3 | This study |
| pATOFM | pBBR1MCS‐2 containing bgla_2g11060 (tofM) gene of BSR3 | This study |
| pATOFR | pBBR1MCS‐2 containing bgla_2g11070 (tofR) gene of BSR3 | This study |
| pATOFIMR | pBBR1MCS‐2 containing bgla_2g11050‐70 (tofIMR) genes of BSR3 | This study |
| pUTOFI | pBBR1MCS‐2 containing bglu_2g14490 (tofI) gene of BGR1 | This study |
| pUTOFM | pBBR1MCS‐2 containing bglu_2g14480 (tofM) gene of BGR1 | This study |
| pUTOFR | pBBR1MCS‐2 containing bglu_2g14470 (tofR) gene of BGR1 | This study |
| pUTOFIMR | pBBR1MCS‐2 containing bglu_2g14470‐90 (tofIMR) genes of BGR1 | This study |
KACC, Korean Agricultural Culture Collection (http://www.genebank.go.kr); KCTC, Korea Collection Type Cultures (http://kctc.kribb.re.kr).