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. 2015 Feb 5;16(7):724–734. doi: 10.1111/mpp.12229

Figure 5.

figure

Resistance assay of transgenic tobacco plants. T0 transgenic TRiV‐AC2 line 3 (A) and TRiV‐AC4 line 4 (B) plants. Plants with the third leaf from the bottom challenged with the agro‐infectious clone of Tomato leaf curl New Delhi virus (ToLCNDV) are shown on the left with untransformed plants shown on the right. (C, D) EcoRI‐digested rolling circle amplification (RCA) products representing viral DNA in the fifth leaf from the bottom at 30 days post‐infiltration (dpi). UT represents the untransformed plant challenged with the agro‐infectious clone. H indicates plant not challenged with ToLCNDV; numbers indicate transgenic plant line numbers. Polymerase chain reaction (PCR) amplification with actin‐specific primers is shown below for normalization. L indicates 1‐kb size marker.