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Quantification of virus in transgenics by quantitative polymerase chain reaction (qPCR). Column graph showing virus accumulation in different T0 transgenic lines [TRiV‐AC2 (A) and TRiV‐AC4 (B)] at 30 days post‐infiltration (dpi), calculated by real‐time PCR, using coat protein primers and β‐actin as an internal control. WT represents wild‐type plant inoculated with virus. The fifth leaf from the bottom was taken for the study. Standard deviations are shown as bars.
