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. 2015 Apr 15;16(9):1000–1005. doi: 10.1111/mpp.12249

Figure 1.

figure

Expression of LmStuA during in vitro growth of Leptosphaeria maculans and oilseed rape infection. The expression of LmStuA was analysed in the strain v23.1.3 by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR); 3, 7 and 14 dpi, RT‐PCR products obtained from RNA isolated from oilseed rape cotyledons (Westar), 3, 7 and 14 days post‐infection; myc, RT‐PCR product obtained from RNA isolated from mycelial culture. RNA extracted from uninfected cotyledons and water was used as a negative control. Gene expression levels are relative to elongation factor 1α (EF1α). Each data point is the average of three biological replicates (extractions from different biological material) and two technical replicates (two RT‐PCRs). Standard error of the mean normalized expression level is indicated by error bars.