Table 2.
Effect of LmStuA silencing on the expression of selected effector genes AvrLm1, AvrLm4‐7 and AvrLm6 in planta
| Isolate/transformant | Mean normalized expression during plant infectiona | ||
|---|---|---|---|
| AvrLm4‐7 | AvrLm1 | AvrLm6 | |
| v23.1.3 | 3.0 | 1.7 | 4.6 |
| s.LmStuA‐21 | 2.9 × 10−3 | b | b |
| s.LmStuA‐49 | 7.6 × 10−2 | 2.3 × 10−1 | 9.8 × 10−2 |
| s.LmStuA‐64 | 1.2 × 10−3 | b | b |
a
Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) analysis of expression was performed in the wild‐type strain v23.1.3 and in three transformants silenced for LmStuA at 7 days post‐infection of oilseed rape leaves. Gene expression levels are relative to elongation factor 1α (EF1α) and calculated as described by Muller et al. (2002). Each value is the average of two biological replicates (two extractions from different biological material) and two technical replicates (two RT‐PCRs).
b
No expression detected.