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. 2014 Jun 30;16(1):48–60. doi: 10.1111/mpp.12160

Figure 3.

figure

Determination of the functionality of predicted nuclear localization signals (NLSs). (a, b) Expression of two 2G02 protein fragments, each containing a predicted monopartite NLS, localized to the cytoplasm. (c) EGFP‐GUS (β‐glucuronidase‐enhanced green fluorescent protein) fused to Arabidopsis histone 2B (At1G07790) was used as a positive control for nuclear import. (d) Following the mutation of three lysine residues in the predicted monopartite NLS of 7H08 to alanine residues, nuclear localization of the effector was still observed. (e) 7H08 also localized to the nucleus following deletion of the predicted NLS. (f) Expression of a 7H08 protein fragment containing the predicted monopartite NLS localized to the cytoplasm.