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. 2014 Jun 30;16(1):48–60. doi: 10.1111/mpp.12160

Figure 5.

figure

Testing of the transcriptional activation activity of 7H08 in yeast. The 7H08 protein was fused with the GAL4 DNA‐binding domain (GAL4BD) and expressed in yeast cells. The ability of yeast colonies to grow on synthetic medium minus tryptophan and histidine (SC/–TH) indicates expression of the HIS3 reporter gene. Expression of the MEL1 reporter gene was determined by the blue coloration of yeast colonies on SC/–T medium with the chromogenic substrate 5‐bromo‐4‐chloro‐3‐indolyl‐α‐d‐galactopyranoside (X‐α‐Gal). A series of deletion variants was created to localize the 7H08 region responsible for transcriptional activation activity.