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. 2014 Jun 30;16(1):48–60. doi: 10.1111/mpp.12160

Figure 6.

figure

Testing of the transcriptional activation activity of 7H08 in plant cells. (a) Schematic diagram of reporter, reference and effector plasmids used in the transient assay. 7H08 was fused to the GAL4 DNA‐binding domain (GAL4BD), whereas the luciferase (LUC) reporter gene was fused to the GAL4 upstream activating sequence (GAL4UAS). (b) Fusion of the transactivation domain of the herpes simplex virus VP16 protein to GAL4BD (GAL4BD‐VP16) resulted in high LUC activity. Data represent the means ± standard deviation from four independent leaf samples. The experiment was replicated three times, and representative results are shown. **P < 0.01. (c) A series of deletion variants of 7H08 was fused to GAL4 and expressed in tobacco leaves to determine the regions of 7H08 able to activate expression of the LUC reporter gene in planta. Data represent means ± standard deviation from four independent leaf samples. The experiment was replicated three times, and representative results are shown. **P < 0.01. (d) Western blot analysis of GAL4BD and GAL4BD‐7H08 deletion series. The anti‐GAL4BD antibody was used to detect the accumulation of GAL4BD‐fused proteins in the leaves of tobacco plants.