Table 1.

Primers and annealing conditions used in this investigation

Primer number	Primer sequence (5′–3′)	Annealing temperature (°C)
1	GACATGAAGCATTCATACCAAATGGG	58
2	GAGCGGCCGCCAGTGTGATGGATATCTGCAAATTACTAGTATTTAGGTGACACTATAGAAAACAAAATAAATAAATAAATATA	61.5
3	ATGGTGGAACAAATAGGCTTCAATGTAACC	61.5
4	TGATTGGGTGAACAAATTGTGTAAGCTACC	61.5
5	TAACCCCTCAATGTGTTGCTTTTGGAGGGC	61.5
6	AAACATTCATACCAAATGGGTGATGAGCTG	61.5
7	CTCACTATAGGGAATATTAAGCTTGGTACCAATTGGTACCACGCGTTTTTTTTTTTTTTTTTTTTTTTTTT	61.5
8	GAGCGGCCGCCAGTGTGATGGATATCTGCAAATTACTAGTATTTAGGTGACACTATAGAAAACAAAACATAACACATAATATC	60
9	GTAATGCCTGGGATTTGCCAGAACCTCCGC	60
10	TATGCCATCCCAATAAGCCACAGGAGGGC	60
11	GTCACCCATTTGGTATGAATGCTTCATGTC	60
12	GGGGATTACGCTGAATTTTGCGGTTGGAC	60
13	GGTAAAGTTTGACCCCTTTTGAATTGGGGAGTTACACAACGAAAACCTCCCGAGAAAAGTGGTTC	58
14	TGATGAGCTGCACAATTACTTAACACCAGATGAAGCTGAACAACACTTCCTTGCTGTTC	58
15	AGAGTCCCACATTACACTTCCTTC	58
16	TGGGTTAGAAGCTGTAGGTTGGTTTTCCATGATTGTTTATTGAAGTTGAT	58
17	ATGGAAAACCAACCTACAGCTTCTAACCCATCAAGTGCACCACCCACAGCC	58
18	ATGGAAAACCAACCTACAGCTTC	58
19	GGCTGGGCTCTGGGCACCAGC	58
20	CTGCTGCTCAAGCTGGTGCCCAGAGCCCAGCCGACTTCTCAAATCCCAATACAG	58