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. 2015 May 12;17(1):16–28. doi: 10.1111/mpp.12258

Figure 2.

figure

Generation and identification of hexokinase gene deletion and FgHXK1 overexpression mutants. (A, B) Homologous gene replacement and complementation strategy for FgHXK1 and FgHXK2, and FgHXK1 overexpression of 2021. (C) Confirmation of FgHXK1 deletion mutants by polymerase chain reaction (PCR) and Southern blot analysis. Probe 1 detected a 2549‐bp fragment in parental strains and complement strains, and probe 2 detected a single band of a 6979‐bp fragment in the two corresponding mutants. (D) Confirmation of FgHXK2 deletion mutants. Probe 3 detected a 3582‐bp fragment in parental strains and complement strains, and probe 2 detected a single band of a 5912‐bp fragment in the two corresponding mutants.