The deletion of MoTUP1 reduced the mycelia growth rate of M. oryzae. A. Growth of Ku80, ΔMotup1 mutants and the complemented strain on CM, MM, SDC and OM media for 7 d. B. Statistical analysis of mycelia growth rate of Ku80, ΔMotup1 mutants and the complemented strain on different media. Three independent biological experiments with three replicates each time were performed and similar results were obtained in each time. Error bars represent the standard deviation, and the double asterisks represent significant difference between Ku80, ΔMotup1 mutants and the complemented strain (p < 0.01).