Figure 2.

(A) Number of nematodes isolated from the stem cuttings at different time points after inoculation. Twenty microlitres of water containing 2000 Bursaphelenchus xylophilus  Ka4 were inoculated on top of the 5‐cm‐long stem cuttings (Fig. 1). The inoculated stem cuttings were incubated at 25 °C and 100% relative humidity. The numbers of nematodes which came through the stem cuttings were counted every 24 h. The number of females observed in each time interval is also shown in the graph. (B) Change in ratio of mucronated (M‐shape) nematodes. Tips of females were observed under a microscope for the same samples as in (A). Nematodes incubated in water or growing on fungi were examined as controls. Error bars represent the standard error of the mean.