Figure 2.

Recombinant Oli‐D1 and Oli‐D2 activate the defence response in Nicotiana benthamiana plants. (A) Accumulation of H₂O₂ in Oli‐D1‐ and Oli‐D2‐infiltrated areas. Recombinant Oli‐D1 and Oli‐D2 at 1 μm were infiltrated into N. benthamiana leaves and in situ detection of H₂O₂ using 3,3′‐diaminobenzidine (DAB) staining was performed on leaves at 4 h post‐inoculation (hpi). (B) Autofluorescence in N. benthamiana leaves induced by Oli‐D1 and Oli‐D2. Recombinant Oli‐D1 and Oli‐D2 at 1 μm were infiltrated into N. benthamiana leaves and autofluorescence was visualized at 48 hpi. Photographs were taken in dark field for green and red fluorescence, in bright field for the morphology of the cells and in combination.