Skip to main content
. 2014 Aug 25;16(3):238–250. doi: 10.1111/mpp.12176

Figure 6.

figure

Oli‐D1 and Oli‐D2 induce resistance in tomato against Botrytis cinerea. (A–C) Resistance induced by recombinant Oli‐D1 and Oli‐D2 proteins. (D–F) Resistance induced by transiently expressed Oli‐D1 and Oli‐D2. (A, D) Representative phenotypes of disease caused by B. cinerea in Oli‐D1‐ and Oli‐D2‐induced plants. (B, E) Size of lesions caused by B. cinerea in detached leaves from Oli‐D1‐ and Oli‐D2‐induced plants at 3 days post‐inoculation (dpi). (C, F) In planta growth of B. cinerea in detached leaves from Oli‐D1‐ and Oli‐D2‐induced plants. One half of the leaf was infiltrated with 1 μm Oli‐D1, Oli‐D2 or a preparation from bacteria carrying pET‐32 empty vector (A–C) or with agrobacteria carrying pGR106‐Oli‐D1, pGR106‐Oli‐D2 or pGR106‐GFP (D–F) and the opposite half of the same leaf was inoculated by dropping 5 μL of spore suspension of B. cinerea at 24 h after infiltration. Photographs, lesion sizes and in planta fungal growth were taken or analysed at 3 dpi. Data presented in (B), (C), (E) and (F) are the means ± SD from three independent experiments. Significant difference at **P < 0.01.