Co‐expression of HRT and Turnip crinkle virus coat protein (TCV CP) induced the hypersensitive response (HR) in Nicotiana benthamiana. (A) HR induction on leaves of N. benthamiana after transient expression of the indicated genes. Photographs were taken at 3 days post‐infiltration (dpi). GFP, green fluorescent protein; +, HR; −, no HR. (B) Electrolyte leakage assay. Measurements of ion conductivity were performed at 3 dpi as in (A). Error bars represent the standard deviation of duplicate measurements calculated from 40 leaf discs (diameter, 1 cm) per construct. Means with the same letter are not significantly different (t‐test, P < 0.05).