Figure 2.

Silencing of the CaBLP5 homologue in Nicotiana benthamiana disrupts the HRT/Turnip crinkle virus coat protein (TCV CP)‐mediated hypersensitive response (HR). (A) HR phenotypes induced by co‐expression of HRT and TCV CP in TRV2‐GFP or TRV2‐CaBLP5‐infected plants (GFP, green fluorescent protein; TRV, Tobacco rattle virus). Samples were photographed at 3 days post‐infiltration (dpi). The numbers indicate Agrobacterium cell concentrations determined from the optical density at 600 nm (OD₆₀₀). (B) Suppression of CaBLP5 homologous gene expression in GFP‐ or CaBLP5‐silenced N. benthamiana plants. Error bars indicate the standard error of three replicates, and asterisks indicate a significant difference from the corresponding control (Student's t‐test, *P < 0.05; **P < 0.005). (C) BiP protein levels in GFP‐ and CaBLP5‐silenced N. benthamiana leaves. Total protein samples were extracted after 21 days of infection. Samples were probed with the indicated antibodies for each protein.