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. 2016 Mar 29;17(9):1382–1397. doi: 10.1111/mpp.12369

Figure 3.

Figure 3

Interaction between HRT and Turnip crinkle virus coat protein (TCV CP) induced the endoplasmic reticulum (ER) stress response. (A) The transcriptional levels of ER stress‐responsive genes following the transient expression of TCV CP or HRT were assayed by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). The expression of Calreticulin1, Beclin1, BiP4/5 and PDI, as markers for ER quality control (QC), and bZIP60, p58IPK and PR4, as markers for ER stress, was analysed at 24 and 36 h post‐infiltration (hpi). Error bars indicate standard deviation (n = 3). Bars with the same letter are not significantly different (t‐test, P < 0.05). (B) BiP protein levels under the HRT‐mediated hypersensitive response (HR) in Nicotiana benthamiana leaves. Total protein was extracted from N. benthamiana leaves expressing the indicated proteins at the designated time points. BiP proteins were analysed by immunoblot analysis using an anti‐BiP antibody.