Figure 4.

Silencing of CaBLP5 reduces the HRT‐ or Rx‐mediated hypersensitive response (HR) to a level similar to that observed after suppression of HSP90 and SGT1. (A) Visual HR phenotypes of the gene‐silenced plants after agroinfiltration to co‐express Turnip crinkle virus coat protein (TCV CP) and HRT, or Potato virus X (PVX) CP and Rx, a resistance protein. Black circles indicate the agroinfiltrated regions. Photographs were taken at 3 days post‐infiltration (dpi). (B) Trypan blue staining at 40 h post‐infiltration (hpi). Green fluorescent protein (GFP) and mutant type CP of PVX were employed as negative reference to TCV CP and PVX CP, respectively. After staining, the leaves were viewed under a light microscope to reveal necrotic plant cells. (C) Electrolyte leakage assay. Ion conductivity was measured at the indicated time points (hpi), and means ± standard deviation were calculated from 60 leaf discs (diameter, 1 cm) per construct; similar results were obtained in three independent experiments, and means with the same letter are not significantly different (t‐test, P < 0.05).