Figure 6.

HRT and Turnip crinkle virus coat protein (TCV CP) were impeded at mRNA levels in BiP4/5‐silenced Nicotiana benthamiana. Total RNA was obtained from leaves of the gene‐silenced plants after expression of either HRT alone or in conjunction with TCV CP at 24 h post‐infiltration (hpi) (white bar) or 41 hpi (dark grey bar). The transcriptional levels of the specific genes were assayed by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Error bars indicate standard deviation (n = 3). Bars with the same letter are not significantly different (t‐test, P < 0.05). Two additional biologically independent experiments yielded similar results.