Figure 8.

Tunicamycin (Tm) treatment blocked the HRT‐mediated hypersensitive response (HR) and HRT accumulation, but increased endoplasmic reticulum (ER)‐resident heat shock protein 70 (HSP70) levels. (A) Verification of HR initiation after Tm treatment. HRT was co‐expressed with Turnip crinkle virus coat protein (TCV CP) in leaves of Nicotiana benthamiana and Tm was infiltrated at a concentration of 10 µm. The images of the infiltrated leaves were photographed under white light or UV illumination at 3 days post‐infiltration (dpi). (B) Effects of Tm treatment on the accumulation of the indicated proteins. Total protein was obtained at 24, 36 and 40 h post‐infiltration (hpi) after expression of haemagglutinin (HA)‐tagged HRT with TCV CP (CP) (C) or green fluorescent protein (GFP) (G). Western blotting was performed with anti‐BiP, anti‐HA, anti‐TCV CP or anti‐GFP antibodies to detect the protein levels.