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. 2016 Feb 8;17(6):875–889. doi: 10.1111/mpp.12337

Figure 1.

Figure 1

TAL14Xam668 is predicted to target many cassava promoters. (a) Target effector binding elements (EBEs) for the transcription activator‐like (TAL) effectors of Xam668 and TAL14CIO151 were predicted using Target Finder (TALE‐NT 2.0) in the 1‐kb cassava promoterome. The predicted target number is plotted (left y‐axis, bar graph) for each TAL effector. The number in each TAL effector name is the number of repeat variable diresidues (RVDs) present in the protein. To visualize the effect of RVD number on EBE prediction, we show the rate at which a correct EBE sequence would occur in the genome at random, where each RVD has a 25% chance of being aligned to its preferred nucleotide (0.25RVD#) (right y‐axis, line graph). (b) The percentage of specific RVDs (NI, NG, HD) for each Xam668 TAL effector is plotted for comparison. (c) EBEs for the first 14 RVDs of TAL15Xam668 predicted as in (a) and plotted alongside the number of predicted targets of TAL14Xam668 and TAL15Xam668 for comparison. (d) The consensus predicted EBE for the top 5000 predicted TAL14Xam668 EBEs in the 1‐kb cassava promoterome, together with the RVD sequence of TAL14Xam668.