Figure 2.

Venn diagram illustrating the RNA‐Sequencing (RNA‐Seq) experimental approach to identify TAL14_Xam668 targeted genes. Cassava leaf tissue was infiltrated with strains at an optical density at 600 nm (OD₆₀₀) of 0.5 and collected after 48 h. RNA‐Seq analysis identified genes up‐regulated [log₂(fold change) >1 and fragments per kilobase transcript per million mapped reads (FPKM) difference >5] by both Xanthomonas axonopodis pv. manihotis (Xam) strain Xam668 compared with mock inoculation (10 mm MgCl₂) and by X. euvesicatoria 85‐10 delivering TAL14_Xam668 [Xe(TAL14)] versus wild‐type Xe as potential TAL14_Xam668 targets. The total number of genes identified in each group is displayed.