Figure 3.

Detection of Eggplant latent viroid (ELVd) RNA by molecular hybridization. Analysis of eggplant RNA preparations by denaturing polyacrylamide gel electrophoresis (PAGE) followed by ethidium bromide staining (a), and northern blot hybridization using an ELVd radioactive probe of − polarity (b). The positions of the monomeric circular (mc) and monomeric linear (ml) ELVd forms are indicated. Additional hybridization bands correspond to oligomeric replication intermediates and degradation products. This analysis shows that samples 1–8, 10 and 12 are infected with ELVd, whereas 9 and 11 remain non‐infected. Lane M, RNA marker with the lengths of the different species indicated on the left in nucleotides.