Table 1.
Translocation of HrpF‐cCya or HopI1‐cCya into Nicotiana tabacum cells by Pseudomonas syringae pv. averrhoi.
| P. syringae pv. averrhoi | Genotype | Assay target* | Translocation † (pmol cAMP/μg protein) |
|---|---|---|---|
| HL1 | Wild‐type | HrpF‐cCya (pNCHU1947) | 32.22 ± 14.19 |
| HopI1‐cCya (pNCHU1881) | 355.18 ± 155.7 | ||
| HL1‐N1033 | ΔhrcC::nptII | HrpF‐cCya (pNCHU1947) | 1.37 ± 0.23 |
| HopI1‐cCya (pNCHU1881) | 1.84 ± 1.9 | ||
| HL1‐N1589 | ΔhrpF | HrpF‐cCya (pNCHU1947) | 2.47 ± 1.16 |
| HopI1‐cCya (pNCHU1881) | 2.33 ± 0.95 | ||
| HL1‐N1589/pNCHU1591 | ΔhrpF/hrpF | HopI1‐cCya (pNCHU1938) | 29.07 ± 14.31 |
| HL1‐N1589/pNCHU1808 | ΔhrpF/hrpF K67A | HopI1‐cCya (pNCHU1938) | 2.16 ± 0.13 |
| HL1/pBBR1MCS‐5 | Wild‐type/EV ‡ | HopI1‐cCya (pNCHU1938) | 354.07 ± 81.55 |
| HL1/pNCHU1591 | Wild‐type/hrpF | HopI1‐cCya (pNCHU1938) | 22.46 ± 16.76 |
| HL1/pNCHU1593 | Wild‐type/hrpG | HopI1‐cCya (pNCHU1938) | 332.92 ± 55.01 |
| HL1/pNCHU1866 | Wild‐type/hrpFG | HopI1‐cCya (pNCHU1938) | 101.40 ± 12.79 |
*Bacterial strains carried pNCHU1947, pNCHU1881 or pNCHU1938 (Table S1) which expresses HrpF‐cCya or HopI1‐cCya from a lac promoter of the plasmid.
†Levels of cyclic adenosine monophosphate (cAMP) in the leaf samples of Nicotiana tabacum were determined at 6 h post‐infiltration with bacterial inoculum at an optical density at 600 nm (OD600) of 0.3, and the values represent the mean and standard deviation (n = 3) in each treatment.
‡EV, empty vector.