Figure 3.

Impairment of heat shock protein 90 (HSP90) function increases the accumulation of HSFA2 in heat‐treated tomatoes; Tomato yellow leaf curl virus (TYLCV) infection mitigates this increase. Immunodetection of the transcription factor HSFA2 in leaf protein extracts following heat shock (HS) and TYLCV infection [uninfected and at 14 and 28 days post‐infection (dpi)]. The OE33 chloroplast protein (OE33) was used as unrelated control. (A) Detached leaves were treated with dimethylsulfoxide (DMSO) (GDA–) and with the HSP90 inhibitor geldanamycin (GDA+) and were subjected to heat shock (HS+) or incubated at room temperature (HS–) for 2 h. (B) Leaves of Hsp90‐ and Sgt‐silenced plants and Tobacco rattle virus (TRV)‐inoculated plants (TRV‐Hsp90 and TRV‐Sgt1; TRV‐0: empty vector) were subjected to HS.