Figure 4.

Silencing of the tomato Hsp90 and Sgt1 genes induces the accumulation of HsfA2 transcripts; Tomato yellow leaf curl virus (TYLCV) infection mitigates the accumulation of HsfA2 and the activation of HsfA2‐dependent gene expression. A quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) approach was used to analyse gene expression in uninfected and TYLCV‐infected (14 days post‐infection, dpi) tomatoes with Hsp90‐ and Sgt1‐silenced genes (TRV‐Hsp90 and TRV‐Sgt1); control plants were inoculated with the Tobacco rattle virus (TRV) vector alone (TRV‐0). For heat shock, the leaves were incubated at 42 °C for 2 h. The relative expression of each gene was calculated in relation to the TRV‐0  TYLCV‐uninfected sample. The results were normalized using the β‐actin gene as an internal marker. Bars represent the average and standard deviation of the relative expression from five independent biological repeats.