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. 2015 Jun 7;17(2):247–260. doi: 10.1111/mpp.12275

Figure 5.

figure

Heat shock protein 90 (HSP90) inactivation results in enhanced accumulation of Tomato yellow leaf curl virus (TYLCV). (A) Quantitative polymerase chain reaction (qPCR) estimation of TYLCV DNA amounts. The results were normalized using the tomato Expressed gene as an internal DNA marker. Bars represent the average and standard deviation of the relative expression from five independent biological repeats. Detached leaflets of TYLCV‐infected tomatoes (21 days post‐infection, dpi) were incubated for 24 h with 0.25 and 0.35 μm geldanamycin (GDA) to inhibit HSP90 [dimethylsulfoxide (DMSO) was used as the control without GDA]; TYLCV DNA amounts were estimated immediately thereafter. (B) Accumulation of TYLCV with time, estimated after 10, 20 and 30 dpi in tomatoes in which Hsp90 and Sgt1 had been silenced (TRV‐Hsp90 and TRV‐Sgt1); plants inoculated with the Tobacco rattle virus (TRV) vector alone (TRV‐0) served as control. (C) Immunodetection of TYLCV coat protein (CP) at 30 dpi in untreated tomato plants (0), in GDA‐inhibited HSP90 (GDA) plants and in tomatoes in which the Hsp90 and Sgt1 genes had been silenced (TRV‐Hsp90 and TRV‐Sgt1); the OE33 chloroplast protein (OE33) was used as unrelated control.