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. 2015 Jun 7;17(2):247–260. doi: 10.1111/mpp.12275

Figure 6.

figure

Heat shock protein 90 (HSP90) inactivation enhances the aggregation of Tomato yellow leaf curl virus (TYLCV) coat protein (CP). Immunodetection of TYLCV CP in tomato leaves (at 21 days post‐infection, dpi) distributed in a linear 10%–50% sucrose gradient; gradients were divided into 10 fractions, 1 (top) to 10 (bottom), and aliquots were subjected to sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). HSP70 was used as a marker of protein loading changes. No CP signal was detected in uninfected Tobacco rattle virus (TRV)‐inoculated tomatoes (o:TRV‐0); aggregated CP patterns were changed in Hsp90 (i:TRV‐Hsp90)‐ and Sgt1 (i:TRV‐Sgt1)‐silenced versus TRV‐inoculated (i:TRV‐0) plants.