Skip to main content
. 2016 Apr 3;17(7):1063–1079. doi: 10.1111/mpp.12348

Figure 2.

Figure 2

Characteristic symptoms on Nicotiana benthamiana systemic leaves after agroinfiltration with a bacterial mixture containing pGC‐RNA1, pGC‐RNA3 and (alternatively) wild‐type (WT) or mutant pGC‐MP constructs at 10 days post‐inoculation (dpi). (A) Symptoms induced by WT and the indicated movement protein (MP) mutants. (B) Close‐up view of systemic leaves from plants infiltrated with pGC‐MPwt, pGC‐MP202 and pGC‐MP137. Note the lack of symptoms for constructs pGC‐MP97, pGC‐MP98, pGC‐MP149, pGC‐MP150 and pGC‐MP169, the necrotic spots and leaf deformation for pGC‐MP202, and the strong mosaic for pGC‐MP137. Plants were agroinfiltrated with a bacterial (Agrobacterium tumefaciens strain C58C1) mixture diluted in agroinfiltration buffer at an optical density of 0.5 at 600 nm. Agroinfiltrated leaves are indicated by an asterisk when visible in the photograph. Scale bars, 1 cm.