Figure 3.

Ourmia melon virus movement protein (MP) and coat protein (CP) accumulation and total protein extracts in Nicotiana benthamiana leaves by Western blot and Coomassie staining. (A) Agroinfiltrated leaves. (B) Upper un‐inoculated leaves from agroinfiltrated plants. (C) Mechanically inoculated (m.i.) N. benthamiana leaves and their source of inoculum [agroinfiltrated leaves (‘agro.’) as in A] consisting of sap prepared from healthy plants or from leaves agroinfiltrated with a bacterial mixture containing pGC‐RNA1, pGC‐RNA3 and pGC‐MPwt, or mutants pGC‐MP253, pGC‐MP97, pGC‐MP98, pGC‐MP149, pGC‐MP150 or pGC‐MP169. Bands at c. 30 and 23 kDa show MP and CP, respectively. Note that a non‐specific band below MP is occasionally visible, probably as a result of cross‐reaction of the MP antibody with plant endogenous proteins, as shown in the healthy control samples.