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. 2015 Jun 29;17(2):272–285. doi: 10.1111/mpp.12279

Figure 4.

figure

Expression analysis of PsHint1‐silenced transformants. Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) evaluation of the expression levels of the gene encoding PsHint1 in hyphae of the initial control strain P6497, the control transformants (CK) and three PsHint1‐silenced transformants (T14, T45 and T22). Relative expression levels were calculated using that of the wild‐type strain P6497 as the reference. All qRT‐PCR experiments were repeated three times using independent RNA isolates.