| Antibodies |
|
|
| Anti-Mouse CD16/CD32 (Mouse BD Fc Block) |
BD Biosciences |
Cat# 553142 RRID:AB_394657 |
| PE anti-mouse CD16/32 antibody |
BioLegend |
Cat# 101308, RRID:AB_312807 |
| Rat Anti-CD45 Monoclonal Antibody, Allophycocyanin Conjugated, Clone 30-F11 |
BD Biosciences |
Cat# 559864, RRID:AB_398672 |
| Rat Anti-Ly-6C Monoclonal Antibody, FITC Conjugated, Clone AL-21 |
BD Biosciences |
Cat# 553104 RRID: AB_394628 |
| PE/Cy7 anti-mouse Ly-6G antibody |
BioLegend |
Cat# 127618, RRID:AB_1877261 |
| CD11b Monoclonal Antibody (M1/70), APC-Cyanine7 |
eBioscience |
Cat# A15390 RRID:AB_2534404 |
| PE anti-mouse/human CD11b antibody |
BioLegend |
Cat# 101207, RRID:AB_312790 |
| PE anti-mouse CD18 antibody |
BioLegend |
Cat# 101407, RRID:AB_312816 |
| PE anti-mouse CD90.2 antibody |
BioLegend |
Cat# 105308, RRID:AB_313179 |
| PE anti-mouse CD369 (Dectin-1, CLEC7A) antibody |
BioLegend |
Cat# 144304, RRID:AB_2561501 |
| APC/Cyanine7 anti-mouse CD45.1 antibody |
BioLegend |
Cat# 110715, RRID:AB_313504 |
| FITC anti-mouse CD45.2 antibody |
BioLegend |
Cat# 109805, RRID:AB_313442 |
| Ly-6G (Gr-1) Monoclonal Antibody (RB6–8C5) |
eBiosciences |
Cat# 14–5931–82, RRID:AB_467730 |
| InVivoMab rat IgG2b isotype control antibody |
Bio X Cell |
Cat# BE0090, RRID:AB_1107780 |
| PE anti-mouse CD64 (FcRI) antibody |
BioLegend |
Cat# 139303, RRID:AB_10613467 |
| Purified anti-mouse CD64 (FcRI) antibody |
BioLegend |
Cat# 139301, RRID:AB_10612757 |
| MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), FITC |
eBiosciences |
Cat# 11–5321–82, RRID:AB_465232 |
| APC anti-mouse CD19 antibody |
BioLegend |
Cat# 115511, RRID:AB_313646 |
| APC anti-mouse CD3 antibody |
BioLegend |
Cat# 100235, RRID:AB_2561455 |
| Mouse EphA2 Phycoerythrin mAb (Clone 233720) antibody |
R&D |
Cat# FAB639P, RRID:AB_2099100 |
| EphA2 (D4A2) XP Rabbit mAb antibody |
Cell Signaling Technology |
Cat# 6997, RRID:AB_10827743 |
| Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb antibody |
Cell Signaling Technology |
Cat# 6347, RRID:AB_11220420 |
| MEK1/2 Antibody |
Cell Signaling Technology |
Cat# 9122, RRID:AB_823567 |
| Anti-MEK1 / 2, phospho (Ser217 / Ser221) Antibody, Unconjugated |
Cell Signaling Technology |
Cat# 9121, RRID:AB_331648 |
| p44/42 MAPK (Erk1/2) Antibody |
BioVision |
Cat# 3085R-100, RRID:AB_10989057 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody |
Cell Signaling Technology |
Cat# 4370, RRID:AB_2315112 |
| PKCdelta (D10E2) Rabbit mAb antibody |
Cell Signaling Technology |
Cat# 9616, RRID:AB_10949973 |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb antibody |
Cell Signaling Technology |
Cat# 2717F RRID: AB_2218658 |
| Syk (D3Z1E) XP Rabbit Antibody |
Cell Signaling Technology |
Cat# 13198 RRID: AB_2687924 |
| Phospho-PKCdelta (Tyr311) Antibody |
Cell Signaling Technology |
Cat# 2055, RRID:AB_330876 |
| p47 phox (phospho-Ser345) antibody |
Biorbyt |
orb126026 |
| Phospho-p47phox (Ser304) Polyclonal Antibody |
Thermo Fisher Scientific |
Cat# PA5–36773, RRID:AB_2553720 |
| p47-phox (A-7) antibody |
Santa Cruz Biotechnology |
Cat# sc-17844, RRID:AB_627987 |
| Mouse IgG (Sepharose Bead Conjugate) antibody |
Cell Signaling Technology |
Cat# 3420, RRID:AB_1549744 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| 7-AAD |
BD Biosciences |
Cat# 559925 |
| 2% heat-inactivated mouse serum |
Gemini Bio-Products |
Cat#100–113 |
| SCH772984 |
Cayman Chemical Company |
Ca# 19166 CAS# 942183–80–4 |
| alamarBlue |
Invitrogen |
Cat# DAL1025 |
| Zymosan (depleted) |
InvivoGen |
Cat# tlrl-zyd Lot# DZN-40–01 |
| Recombinant Murine M-CSF |
Peprotech Inc |
Cat# 315–02 |
| H2DCFDA |
Invitrogen |
Cat# D399 |
| Critical Commercial Assays |
|
|
| MojoSort Mouse Neutrophil Isolation Kit |
BioLegend |
Cat# 480058 |
| Luminex Mouse Magnetic Assay |
R&D |
Cat# LXSAMSM |
| Experimental Models: Organisms/Strains |
|
|
|
C. albicans SC5314 |
Fonzi and Irwin, 1993 |
N/A |
|
C. albicans-GFP |
Levitin et al., 2007 |
N/A |
|
S. aureus RN6390 |
Kahl et al., 2000 |
N/A |
|
Epha2trn1Jrui/J |
Finney et al., 2017 |
N/A |
| C57BL/6J |
The Jackson Laboratory |
RRID: IMSR_JAX:000664 |
| B6.129S7-Itgb2trn1Bay/J |
The Jackson Laboratory |
RRID:IMSR_JAX:002128 |
| B6.129P2-Fcer1gtrn1Rav N12 |
Taconics |
RRID:IMSR_TAC:583 |
| Software and Algorithms |
|
|
| FlowJo V10 |
Treestar |
https://www.flowjo.com/ |
| GraphPad Prism V8 |
GraphPad |
https://www.graphpad.com/ |
| Other |
|
|
| Luminex multiplex analyzer |
Luminex |
https://www.luminexcorp.com |
| BD LSR II |
BD Biosciences |
https://www.bd.com |
| Leica TCS SP8 Confocal system |
Leica |
https://www.leica-microsystems.com |
