Table 3.
Primers used for quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR)
| Microarray spot ID | GlymaID | Short annotation | Forward primer | Reverse primer |
|---|---|---|---|---|
| B020384 | Glyma01g42660 | PR5 | aggcatcagggcacctctcctt | ttcctggcggctgcaacaac |
| AI494656 | Glyma01g42670 | PR5 | taagggcacctgggggatgcaa | cgaccttgtagttagagccagcgg |
| AI855519 | Glyma01g36620 | Sulphurtransferase | ttgatgtctctgccacttgc | aacttgggtggaacagcaac |
| BI424301 | Glyma02g04820 | Class I chitinase | ggctctttcaatggctttgg | acccacctgtggtttcatgag |
| BQ297212 | Glyma02g07180 | Chlorophyll‐binding protein | ctggtggtgcttttgatcct | cacaaaagccaacaaagcaa |
| AW458674 | Glyma03g21610 | MAPK kinase | agagcttggggatggttctt | ttgaccgcgacaatctcata |
| BE020966 | Glyma05g29400 | Glutathione S‐transferase | tcgatacagataattgacccattga | cttgatcacaggatggctaagaaa |
| BI786214 | Glyma05g29400 | Glutathione S‐transferase | tcgatacagataattgacccattga | cttgatcacaggatggctaagaaa |
| AI442353 | Glyma06g07170 | Serine–threonine protein kinase | gccttgctttgttcttccac | tcaggcaacctttgctttct |
| BG550911 | Glyma06g45450 | Diacylglycerol kinase | catggaactcgtcttgctca | aaggttccccatcaatcctc |
| AI441522 | Glyma07g39020 | Peroxidase | gccctggagttgtttcatgt | accacatcagctctgctcct |
| AW132407 | Glyma08g06820 | NAD‐malate dehydrogenase | tttccaggaagggagtgatg | gcaaagaaagggattgacca |
| AI939107 | Glyma08g07540 | ABC transporter | aaccaggatgctgatgaagg | tcttcttcttcccacatgcac |
| AI496388 | Glyma09g02600 | Peroxidase | gcgtggtttcttgtgctgat | caagggttcgatttgctgtt |
| AW133469 | Glyma09g28200 | Light‐harvesting complex 5 | gatgggggtacgctgaacta | gcacctcccacaagaacaat |
| AI441060 | Glyma09g30300 | MAPK kinase | cgccggagtttcacgatt | acctctccccggactcctt |
| AI443042 | Glyma10g32820 | Protease inhibitor | ggtaagagctcatggcctgagt | aaaccctatcacatcggaaatca |
| AI441937 | Glyma11g01350 | Chalcone synthase | cacatgaccgagctcaaaga | catgtcttgcctagcatcca |
| BI972951 | Glyma11g10080 | Glycosyl hydrolase | ccagcacttgaaaacattcagaa | tgtctattgctgttgacaccttcat |
| AI496533 | Glyma11g29460 | Cinnamoyl CoA reductase | gactttgcgaaggagacagg | taggtctctttgcccccttt |
| AW570395 | Glyma11g29460 | Cinnamoyl CoA reductase | gactttgcgaaggagacagg | taggtctctttgcccccttt |
| BG550897 | Glyma13g16950 | OPDA reductase | caggtagaggcatggagaaatgta | aacatgccagagttgacagaagata |
| AI443120 | Glyma13g26200 | Cytochrome p450 | ggagaggttgttgagggtga | agtctgttcctgccgagaaa |
| AW507727 | Glyma13g41110 | Envelope protein | gctggcgtttatgttggaat | ttgttgctggctgcagatac |
| AI736217 | Glyma15g05790 | Light‐harvesting complex 6 | ctgctgcaccaaagaagtca | ccaaggcctagtggatcaaa |
| AW397279 | Glyma15g43110 | Photosystem II protein | tcctctggtccattctttgg | ttggcagcattcactacgag |
| AW761087 | Glyma16g34570 | Aldo/keto reductase | aaggcaaaagggaagaccat | ggttctgcttcatcctctcg |
| BI788114 | Glyma17g01720 | Cationic peroxidase | gccagagatggcattgtttc | cattgtggtctgggaggaac |
| AW761241 | Glyma17g03350 | Bet v I family protein | catcccaaaggctgtcgaa | gccaccgttcccctcaa |
| AW101559 | Glyma18g06510 | Dihydroflavonol reductase | ttatgccaagccctaactgg | caaccccttctgtttgcagt |
| AI901207 | Glyma18g06510 | Dihydroflavonol reductase | ttatgccaagccctaactgg | caaccccttctgtttgcagt |
| BQ080089 | Glyma18g16780 | TIR‐NBS‐LRR‐type protein | agggagcaatccaatcgtaaga | ggccacaatttgttacggttaca |
| AW396185* | Glyma10g38456 | Cons15 | taaagagcaccatgcctatcc | tggttatgtgagcagatgcaa |
*Constitutive control cons15 used for data normalization (Libault et al., 2008).
LRR, leucine‐rich repeat; MAPK, mitogen‐activated protein kinase; NBS, nucleotide‐binding site; OPDA, 12‐oxophytodienoate; PR5, pathogenesis‐related 5; TIR, Toll/interleukin‐1 receptor.