Figure 4.

Quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR) confirmed the regulation of 14 genes from the microarray results. The expression profiles of these genes were determined by qRT‐PCR at 6, 8 and 24 h post‐treatment (hpt). Expression levels are given as the log₂‐transformed fold change of Fusarium virguliforme phytotoxin (Fv toxin)‐treated soybeans compared with mock treatment. In each histogram of qRT‐PCR results, the means of three biological replicates are represented ± standard deviation (SD). Bars from left to right: white bars, PI567.374; grey bars, Williams 82; striped bars, Essex. The soybean actin gene was used as a reference control gene.