Skip to main content
. 2013 Mar 4;14(3):265–278. doi: 10.1111/mpp.12002

Figure 1.

figure

Silencing suppression activity of Chinese wheat mosaic virus (CWMV) cysteine‐rich protein (CRP) in Agrobacterium co‐infiltration assay. (A) Green fluorescent protein (GFP)‐transgenic Nicotiana benthamiana line 16c leaves were infiltrated with mixtures of Agrobacterium culture harbouring pBin‐GFP plus Agrobacterium harbouring the construct indicated [CWMV CRP, Potato virus Y (PVY) HC‐Pro or Tomato bushy stunt virus (TBSV) p19]. GFP fluorescence was visualized under long‐wavelength UV light and photographed 3, 4 and 5 days after inoculation (dai). (B) Northern blot analysis of GFP mRNA accumulation in agroinfiltrated patches of leaves shown in (A). The RNA gel was stained with ethidium bromide and 28S rRNA is shown as a loading control (bottom panel). (C) Northern blot analysis of GFP siRNA accumulation in agroinfiltrated patches of leaves shown in (A). The RNA gel was stained with ethidium bromide and 5S rRNA and tRNA are shown as loading control (bottom panel).