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. 2013 Mar 4;14(3):265–278. doi: 10.1111/mpp.12002

Figure 7.

figure

Effects of the expression of wild‐type and mutants of Chinese wheat mosaic virus (CWMV) cysteine‐rich protein (CRP) on Potato virus X (PVX) symptoms and accumulation. (A) Schematic representation of a PVX vector (pGR106 base) chimera carrying CWMV CRP or mutant genes (not to scale). (B) Nicotiana benthamiana plants infected with PVX vector chimeras carrying the wild‐type, antisense (as) or mutants (C20A, ΔC9, ΔC19 and ΔC40) of CWMV CRP. Plants were photographed at 14 days after inoculation (dai). (C) Western blot analysis of PVX coat protein (CP) accumulation in the upper systemically infected leaves of N. benthamiana. Total protein was extracted from leaves at 10 dai. Coomassie blue (CB)‐stained total cell proteins are shown as loading controls (bottom panel).