Figure 4.
Growth in planta and symptom induction ability of the lipA mutant. (A) Growth in planta of the lipA mutant (lipA –) relative to the wild‐type strain (wt) in tomato Hawaii 7998 (H7998) (left) and Micro Tom (right) following dip inoculation with 5 × 105 colony‐forming units (cfu)/mL bacterial suspensions. Results represent averages and standard deviations (SDs) of one experiment (of three or two with similar results for cultivars H7998 and Micro Tom, respectively). In each experiment, five replicates (each composed of five leaf discs) were used per treatment/time point. Asterisks represent significant differences between strains (P= 0.05) at the given time point according to Student's t‐test. (B) Symptom induction of the lipA mutant compared with the wild‐type strain in tomato H7998 following syringe infiltration with bacterial suspensions of 106 cfu/mL. In each experiment (three with similar results), each strain was used to inoculate at least 30 leaflets. The photograph was taken 7 days after infiltration.