Figure 6.

Growth of Xanthomonas campestris pv. vesicatoria (Xcv) strains in a medium containing olive oil as sole carbon source with gentamicin (Gm), including (A and B) or not including (C) neutral red. Strains were the lipA mutant and the wild‐type, both carrying pBBR1MCS‐5 (lipA ^–/pBBR1 and wt/pBBR1, respectively), and the lipA mutant carrying pBBR1‐lipA (complemented strain, lipA ^–/comp). (A) Photograph taken after 3 days of growth at 28 °C with shaking. In addition to enhanced growth, a stronger red coloration in the wild‐type and the complemented strain relative to the mutant indicates enhanced medium acidification as a consequence of higher lipolytic activity. (B) Quantification of lipolytic activity by measurement of the optical density of the cultures at 595 nm (OD₅₉₅). Results represent averages and standard deviations (SDs) (three replicates per treatment) of one experiment of three with similar results. Different letters indicate statistical significance at P= 0.05 according to Tukey–Kramer test and analysis of variance (anova). (C) Growth curves of the strains in olive oil medium without neutral red. Each point represents the average and SD of three replicates of one experiment (of two with similar results).