Table 1.
Strains and plasmids used in this study.
| Strains and plasmids | Relevant characteristics* | Reference or source |
|---|---|---|
| Xanthomonas campestris pv. vesicatoria | ||
| 97‐2 | Wild‐type, Xanthomonas perforans according to the new nomenclature; belongs to race T3 | Astua‐Monge et al. (2000) |
| XcvRes | KmR; reporter strain containing the res cassette inserted within hpaG in 97‐2 | Tamir‐Ariel et al. (2007) |
| ResPlipA | KmR, GmR; PlipA (lipA promoter)::tnpR::uidA in XcvRes background | This work |
| ResPlipArev | KmR, GmR; PlipArev (lipA promoter in reverse orientation)::tnpR::uidA in XcvRes | This work |
| ResPhrpA | KmR, CmR; PhrpA (hrpA promoter)::tnpR::uidA in XcvRes | Tamir‐Ariel et al. (2007) |
| lipA mutant | KmR; strain 97‐2 disrupted in the lipA open reading frame (ORF) by insertional mutagenesis with plasmid pJPlipA | This work |
| lipA –/comp | KmR, GmR; lipA mutant carrying plasmid pBBR1‐lipA (complemented strain) | This work |
| lipA –/pBBR1 | KmR, GmR; lipA mutant carrying plasmid pBBR1MCS‐5 | This work |
| wt/pBBR1 | GmR; wild‐type 97‐2 carrying plasmid pBBR1MCS‐5 | This work |
| Escherichia coli | ||
| S17‐1 λ pir | λ lysogenic S17‐1 derivative producing π protein for replication of plasmids carrying oriR6K; recA pro hsdR RP4‐2‐Tc::Mu‐Km::Tn7 λ‐ pir | Simon et al. (1983) |
| Plasmids | ||
| pJP5603 | KmR; R6K‐based suicide vector; requires the pir‐encoded π protein for replication | Penfold and Pemberton (1992) |
| pJPGm | GmR; a pJP5603‐derived vector, in which the KmR gene was replaced by a GmR gene, excised from pML122 (Labes et al., 1990) | This work |
| pJPGmtnpR | GmR; pJPGm carrying a promoterless tnpR::uidA | This work |
| pPlipA | GmR; pJPGmtnpR with the lipA promoter cloned upstream and in the same orientation as tnpR::uidA to create a transcriptional fusion | This work |
| pPlipArev | GmR; pJPGmtnpR with the lipA promoter cloned upstream and in the opposite orientation to tnpR::uidA | This work |
| pJPlipA | KmR; pJP5603 carrying a 614‐bp internal fragment of the lipA ORF; used to generate the lipA mutant by insertional mutagenesis | This work |
| pBBR1MCS‐5 | GmR; RK2 broad‐host‐range cloning vector | Kovach et al. (1995) |
| pBBR1‐lipA | GmR; pBBR1MCS‐5 carrying wild‐type (97‐2) lipA with its native promoter region | This work |
*
In strains and plasmids, KmR, CmR and GmR represent kanamycin, chloramphenicol and gentamicin resistance, respectively.