Figure 4.

Laser scanning confocal fluorescence images (top panel) of H₂O₂ accumulation in Metschnikowia fructicola cells, combined with bright field images (bottom panel). Yeast was grown in nutrient yeast dextrose broth (NYDB) for 24 h (A) or 48 h (B) at 25 °C. Scale bar, 50 µm. Quantification of H₂O₂ level in yeast cells, expressed as the relative pixel intensity (rpi) (C). Histograms represent the mean ± standard error (n= 6). Histograms with different letters indicate significant differences according to Tukey's test (P < 0.05).