Figure 3.
Verification of microarray data by quantitative real‐time polymerase chain reaction (qRT‐PCR). The relative fold change of each gene was derived from the comparison of the compound‐treated versus dimethylsulphoxide (DMSO)‐treated Erwinia amylovora strain Ea273 in hrp (hypersensitive response and pathogenicity)‐inducing minimal medium (HMM) for compounds 3 and 9 using qRT‐PCR. The 16S rDNA (rrsA) gene was used as a control. The values of the relative fold change were means of three replicates. The experiments were repeated three times with similar results. Error bars indicate the standard deviation.