Figure 6.

Transient assays of four elicitins from Phytophthora phaseoli showing the induction of hypersensitive response (HR)‐like host cell death. (A) Leaves of Nicotiana benthamiana were infiltrated with Agrobacterium tumefaciens (strain GV3101) to express candidate elicitins. Agrobacterium containing INF1 from P. infestans (PGR106INF1) was used as a positive control, and pCambia 1301s and PGR106GFP were used as negative controls. (B) Agrobacterium‐infiltrated leaves from (A) were stained with trypan blue. This experiment was performed with at least three biological replicates and these are representative images taken at 7 and 18 days post‐inoculation (dpi).