Figure 5.

The nlxA gene is involved in swimming and swarming motility. Two microlitres of bacterial solution [10⁸ colony‐forming units (CFU)/mL in nutrient broth (NB)] were inoculated on the swimming assay (0.3% agar) (A, B) and swarming assay (0.7% agar) (C, D) plates. The bacterial motility was measured at different time points as indicated. The movement of bacterial cells was photographed at 5 and 8 days post‐inoculation (dpi) on the swimming and swarming plates, respectively. The experiment was repeated three times with three replicates each time. Means and standard errors of three replicates from one representative result are shown. Vertical bars represent the standard errors of the means.